Lipid accumulation in beige adipocytes treated with rosiglitazone or diosmin was detected by Oil Red O staining and measured at 520 nm by SpectraMax 190 microplate reader (Molecular Devices). The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Find regional support. com. The multi-channel design truly mimics a dual-beam spectrophotometer. The absorbances were measured at 450 nm and 630 nm on a SpectraMax 190 microplate reader (Molecular Devices) or an SH-1200Lab microplate reader (Corona Electric). 118 Gemini XPS Fluorescence Microplate Reader . Gemini™. Also refer to this Multimode Reader Comparison Chart. (a) Chemical structures of 1 (HADP) and its analogues 2–4. Protein concentrations were calculated using. Small volumes (5 μL, 50 mM) of compound solution, dissolved into DMSO, were added to the. Wavelength accuracy ±1. Thông Số Kỹ Thuật Máy Quang Phổ Định Lượng DNA/RNA SpectraMax Quickdrop - Khoảng bước sóng: 190 -1100 nm - Thể tích nhỏ nhất: 0. Optimized for fluorescence microplate readers. Synthesis and crystallization of complex 1. Measuring Instruments Molecular Devices SPECTRAmax 190 Operator's Manual. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Read Cuvette - For the SpectraMax Plus 384, press to initiate the sample read of the cuvette. Part # 0112-0085 Rev. Brand: MOLECULAR DEVICES. Temperature control. Protein concentrations were calculated using polynomial 2nd analysis from the protein standards. Total flavonoid content The total flavonoid content of the extracts was determined according to the method described by Herald et al. 0 nm. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. The mixture was shaken and incubated for 30 min at room temperature in the dark. Molecular Devices SpectraMax 190 and SoftMax Pro6. Examples: “Press carefully to engage,” “ Do not. Do not use a dry plate. The corrected absorbance is within 5% of the. The ability to detect 16 ng/well of DNA and to quantitate 50 ng of DNA provides the same sensitivity as a spectrophotometer. Add to cart. SpectraMax® 340PC384 Microplate Reader for acquiring data in endpoint, kinetic, and spectral scan modes using wavelegths from 340-850 nm, tunable in 1 nm increments. The absorbance was measured using ELISA analytical instruments SpectraMax 190 (Thermo Fisher Scientific, CA, United States) at a wavelength of 450 nm. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13SpectraMax 190 Absorbance Microplate Reader. The absorbance was determined at 450 nm with SpectraMAX-190 ELISA reader (Molecular Devices, Sunnyvale, CA). 1. Plate chamber design of the SpectraMax category 1 microplate readers. 5 - 2. The PathCheck sensor can extend the instrument linearity by a factor of three or more. mg −1 protein using the extinction coefficient of 39. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Manufacturer: Molecular Devices; Model: SpectraMax 190 * Wavelength Selection Monochromator, tunable 1. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wella SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). The patented multichannel optical design of the SpectraMax 190 Absorbance. The SpectraMax 190 reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The cooled mixture was centrifuged at 4000 rpm for 10 min, and the supernatant was collected. Here, we demonstrate several different ways the ABS Plus reader, in combination with the SpectraDrop™ Micro-Volume Microplate and SoftMax® Pro Software, can quantitate double-stranded DNA and bovine serum albumin (BSA. * dsDNA sensitivity specified for the following instruments: SpectraMax® 190 Readers, SpectraMax® Plus384 Readers,. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. We used three technical replications for each biological replication and measured absorbance at 595 nm using a SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 2) If you wish to regulate the temperature inside the microplate reading cham-. It measures the optical density (OD) of samples in 96-wellSpectramax M Series, supplied by Danaher Inc, used in various techniques. . 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. The SpectraMax® 190 Microplate Reader from Molecular Devices is ideal for UV-Vis life science applications, especially DNA analysis. This technical note is designed to provide information . In vitro blood test. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- StakMax Handling Stacker Base Unit. Example: [Drawer]. spectramax plus 384 microplate reader, pathcheck sensor, spectramax plus 384 reader, softmax pro software, stakmax microplate stacker, spectratest validation package, microplate. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. . 5mm filters. SpectraMax 190 Absorbance Microplate Reader. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 1. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. The . Fluorescence intensity was measured using a. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. Absorption was measured at 450 nm 24 h after premix added using SpectraMax 190 (Molecular Devices, LLC, Sunnyvale, CA, USA) to produce a cell growth curve. Type: Clear: Sale. The equation that allows one to calculate absorbance from % transmittance is. 16 $ $ 36,002 . The SpectraMax Plus 384 Microplate Spectrophotometer is a versatile and reliable instrument that can measure absorbance in the UV and visible range. com always has the largest. 7. fl 312 gain calibration failed. Contact AnytimeLabTraderThe OD values of this chromogenic enzymatic reaction were read on a microplate spectrophotometer (Spectramax 190, Molecular Devices Corporation, Sunnyvale, CA) at 450 nm within 30 min after adding the stop solution. The PathCheck sensor can extend the instrument linearity by a factor of three or more. SpectraMax 190 Absorbance Microplate Reader with laptop and software Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. The VersaMax and SpectraMax 190 read 96-well microplates. All samples were tested in duplicate and the optical density values were read on a microplate spectrophotometer (Spectramax 190, Molecular devices Corporation, CA, USA) at 450 nm. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. 3. The SpectraMax® 190 UV-Vis Microplate Spectrophotometer from Molecular Devices is ideal for life science applications, especially DNA analysis. Reads various kinds of 96-wells plates, and is equipped with shaking function. 1) Turn on the power switch of the SPECTRAmax 190 (located on the back panel). . to View Figures. . This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. The SpectraMax 190 reader is the tool for DNA analysis. We used three technical replications for each biological replication and measured absorbance at 595 nm using a SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). ®System (PerkinElmer) On The SpectraMax ID5 Reader. Chitin weight was plotted against corresponding absorbance values resulting in a standard curve. The SpectraMax® ABS Plus is a compact UV-Vis absorbance microplate reader ideal for these types of quantitative assays. Find many great new & used options and get the best deals for Molecular Devices SpectraMax 190 Absorbance Microplate Reader at the best online prices at eBay! Free shipping for many products!An ELISA reader (SpectraMax 190) was used to measure the absorbance of formazan solution at 490 nm. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Category 2 and 3 microplate readers use gradient heating as well. Wavelength precision. 0 nm increments * Wavelength Bandwidth 2 nm * Wavelength Accuracy < ± 2. 034 g, 0. ATP standard curves read on SpectraMax microplate readers. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. 0 out of 5 $ 4,499-5%. SpectraMax® Plus 384. Cell Apoptosis Assay Following treatment, the apoptosis rate of H9c2 cells was analyzed by flow cytometry analyses using the Annexin V-FITC/PI Cell Apoptosis Detection Kit (Dojindo. The plate. The minimum detection limit of the assay was 18. 05,. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. SpectraMax 190酶标仪适用于大多数生命科学研究工作，尤其是DNA分析。能够检测任何标准96孔或UV-透射96孔微孔板。内置单色器，连续波长范围为190-850nm,步进波长1nm。提供终点检测、动力学和光谱扫描三种测读模式。具有温控系统和微孔板震荡混. The SPECTRAmax 190 can accommodate standard 96-well microplates, strip wells, and Þlter-bottom microplates. Multimode Reader Comparison Chart. Photometric accuracy. The ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. Currently unavailable. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The SpectraMax® and VersaMax™ require proper ventilation to keep their internal components cool. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. Published Dec 16, 2020 | Updated Jan 25, 2021 Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax. Free shipping. The absorbance was read every 30 s for a period of 3 min using a SpectraMax 190 microplate reader. 2 - 3. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. For very concentrated samples, use aSpectraMax iD3 reader. 00 . Three kits covering a wide range of dsDNA concentrations. SpectraMax iD3 and iD5 Register or Sign In. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. GM2000 Register or Sign In. , 0, 0. Spectramax 190 Absorbance Microplate reader, included validation report, control software, 190-850 nm, Fully inspected and validated with Warranty. Temperature uniformity within the microplate may vary depending on the type of microplate used. Upgradeable modules are also available including western blot, cell imaging, and fast. 0 nm. Streamline GLP/GMP compliance. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The cell pellets were lysed with 200 mmol/L NaOH, and the iron content was determined using the ferrozine assay. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Description 1. The temperature in the plate chamber is ambient and isothermal. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. 01, 0. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 1 in stock. Ltd. Example: [Drawer]. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. EZ-Cytox (10 µL) was added to each of the wells of the plate and incubated for 1 h. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Features. Packed 25 plates per sleeve, 2 sleeves per case. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. Eliminate the need for purchasing and changing out filters for wavelength optimization with a monochromator-based wavelength selection for UV-visible absorbance between 190 nm and 850 nm Read up to 6 wavelengths in endpoint or kinetic mode for 96-well plates, and perform spectral scans to determine wavelength peaksIMI detection was carried out using the Eurofins Imidacloprid ELISA kit (Eurofins Abraxis, Warminster, PA, USA) following the manufacturer’s instructions for extraction and quantification of imidacloprid residue in leaf tissue and using a spectrophotometer (SpectraMax 190; Molecular Devices, San Jose, CA, USA). A = 2 - log 10 (%T). 0 nm increments Wavelength bandwidth 2 nm Wavelength accuracy ±1. The optical density was measured at 540 nm using a SpectraMax 190 microplate reader (Molecular Devices, San Jose, CA, USA). VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Read Cuvette - For the SpectraMax Plus 384, press to initiate the sample read of the cuvette. After this date, all service and support will be on a reasonable effort basis At this time, we are happy to help you upgrade to the SpectraMax® ABS or ABS Plus Microplate Readers which containThe solubility of compounds was measured using a SPECTRAMAX 190 (Molecular Devices, San Jose, CA, USA). SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentra-tions. The SPECTRAmax 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Near bottom. Sale Price: $12,125. , Tokyo, Japan) was added to each well every 24 h. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual 1-3 Introduction General Overview The SPECTRAmaxŽ 190 microplate spectrophotometer provides rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Since this is an older reader, we recommend that new users choose our newer model i3 reader. Prepare your iD series instrument for shipment. ¨The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. Italic and boldface type are used for emphasis. The following steps provide a quick reminder of the basic operating proce- Overview dures required to read a microplate using the SPECTRAmax 190. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. $500. The use of gene reporters such as luciferase permits highly sensitive and nondestructive monitoring of gene expression. The SPECTRAmax 340PC can mea-The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed . SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. ; Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. Example: [Drawer]. D 1 1. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The data are shown in optical density (O. Examples: “Press carefully to engage,” “Do not. • Last day to purchase a service plan for SpectraMax 190 Microplate Reader is December 31, 2027. Our Price: $10,995. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-The SPECTRAmax 190 is equipped with an 8-pin DIN RS-232 serial port through which a computer can communicate with and control the instrument. Table 1. 306 plate air cal fail,low light. Examples: fiPress carefully to engage,fl fiDo not press down. SpectraMax 190 (Serial number starting with N) SpectraMax 250 SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number. Determine concentration using the Beer-Lambert LawThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well a SpectraMax 190 spectrophotometer (Molecular Devices, LLC, Sunnyvale, CA). Seller InformationAnytimeLabTrader. Three-dimensional Matrigel culture. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Fluorescent Polarization: Solid black plates, as above. Offer Ends In: 38 Days 16: 07: 54. The stability of the luminescent signal, as. Microplate Reader, For Lab. TECHNICAL NOTE. qPCR HBV DNA was extracted as. Prior to the reading of each plate, the microplate reader was “blanked” with a. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. ” We would like to show you a description here but the site won’t allow us. Performance Specifications . The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. A bandwidth of 5 nm or less is required for accurate absorbance measurement of DNA 1. The drawing shows a standard microplate of 15 mm height covered with a lid. The protein concentration was measured by an enhanced BCA protein assay kit (Beyotime; Nanjing, China). The ELISA signal was measured using the ELISA plate reader SpectraMax 190 (Molecular Devices, Sunnyvale, CA, USA). To provide access to disconnect power from the instrument, maintain a 20 cm to 30 cm (7. SpectraMax 190 Absorbance Microplate Readerwith laptop and softwareEnables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the. SpectraMax 190 and Spect FRET fluorescence, top- le-Mode Detection Fluorescence Sir and bottom-read, cell-based assay, lal injection in both 96- and 384-well lates, excellent sensitivity, Autorinse range, du microp 0+ pre-defined protocols; lac compatible based (visible 405-750 nm)The obtained mixture was reacted in a boiling water bath for 20 min, and then, cooled down immediately. UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. aliquot size needed for sequencing)We would like to show you a description here but the site won’t allow us. The SpectraMax 190 reader is the tool for DNA analysis. The VersaMax and SpectraMax 190 read 96-well microplates. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. Molecular Devices microplate spectrophotometer, mod SpectraMAX 190, s/n 02552, includes computer. Microplate Spectrophotometer Operator’s Manual. Molecular Devices SpectraMax 190 User Manual (55 pages) Microplate Spectrophotometers. TheSpectraMax® SpectraMax 190 (Serial number starting with N) SpectraMax 250 SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. Simplify and expedite experiments with SoftMax Pro Software’s. Temperature control and shaking come as standard. 0 nm increments . 2 nm. Examples: “Press carefully to engage,” “ Do not. For the GL261 and U87 mouse models, tumor tissue and the contralateral normal brain tissue (non-tumor cells injected hemispheres) was collected as described below and analyzed with the TNFα ELISA kit. SpectraMax ID5 Plate Reader (Molecular Devices) DescriptionCrystal violet was then dissolved using a 10% acetic solution, and the absorbance was read using SpectraMax 190 Microplate Reader (Molecular Devices) at 570 nm. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. High tolerance for contaminants. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. Microplate Readers Promega GloMax Plate Reader Multi-Detection. The ability to detect 16 ng/well of DNA and to quantitate 50 ng of DNA provides the same sensitivity as a spectrophotometer. All the measurements were taken in triplicate and the mean values were calculated. A microplate reader for endpoint, kinetic, and spectral scan assays at UV-visible wavelengths, with temperature control, shaking capabilities, and multichannel optical design. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. Analysis of pro-inflammatory macrophage phenotypic. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Monochromator, tunable 1. For very concentrated samples, use a minimum volumeSPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Product Description. The Spectramax 190 has an array of data collection modes, including kinetic, endpoint, and spectral scan modes. It measures the optical density (OD) of samples in 96-wellPreconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. SpectraMax 190 Absorbance Microplate Reader from Molecular Devices LLC. 1) Select the correct plate and the wells that you want to measure. Protein concentrations were calculated using polynomial 2nd analysis from the protein standards. ®The SpectraMax Glo Steady-Luc Reporter Assay Kit is designed for optimal performance on microplate readers with high throughput enabled by both the microplate format and extended signal window. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 2 nm 光度量范围 0-4. For very concentrated samples, use a minimumSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Molecular Devices SpectraMax 340PC384 Microplate Reader $ 4,000. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Tumor cells were plated onto 96-well plates (500 cells/well), and 10 µl of Premix WST-1 Cell Proliferation Assay System (Takara Bio Inc. Wavelength Range: 190 - 850 nm. 006 O. Type: Clear: Add to Cart. 190-SC - SpectraMax 190 Microplate Reader with 1-Year Service Contract - SpectraMax 190 Absorbance Plate Reader, Molecular Devices - Each מותג: Molecular Devices 36,002. The ability to detect 16 ng/well of DNA and to quantitate 50 ng of DNA provides the same sensitivity as a spectrophotometer. no. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 078 μg/mL), and the results were expressed as μg Fe (II)/g of extract. spectramax i3x (Danaher Inc) Danaher Inc is a verified supplier. HI Wendy, I dont have a lot of expertise but I'd just suggest you to check that the filter wheel isn't touching the metal chassis. . Learn more about Services for SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. The multi-channel design truly mimics a dual-beam spectrophotometer. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. Optional tools for verifying the SpectraMax 190 reader performance include the SpectraTest ABS1 Absorbance Plate and IQ/OQ/PQ manual. Find many great new & used options and get the best deals for Molecular Devices SpectraMAX 190 Microplate Reader at the best online prices at eBay! Free shipping for many products!Mini-Bead Beater (BioSpec Products); fluorimeter microplate reader (SpectraMax. For aqueous solutions, our patented* PathCheck® sensor. TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive measurementsofavarietyofanalytesacrossawiderangeofconcentrations. Very small footprint suitable for tight space requirements. A = Log 10 (I 0 /I). What is the part number for the filter wheel motor? I am looking to replace mine because I suspect it is the cause of the failure• SpectraMax® 190 (Serial#-NNR-xxxxx) •ersaMax™ (Serial#-BNR-xxxxx) V • AquaMax® Microplate Washer. SpectraMax® 190. A wide linear dynamic range ensures accurate assay results across a broad span of sample types. Select the Driver tab and click Update Driver. DNA provides the same sensitivity as a spectrophotometer. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. See More Information. Wavelength Selection . The 3,3′,5,5′-tetramethylbenzidine (TMB) substrate was added after the incubation. 00 +$350. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS control panel are shown in boxed Helvetica type. 6. #655801; Quartz: Hellma cat. Quercetin was used as the standard (1 mg/ml) solution in order to obtain the calibration curve using a series of concentrations, i. Protein concentrations were calculated using. min −1. Service Contracts, Preventative Maintenance and Performance. These models can no longer be repaired. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Flavonoid Compositions Analysis . Call 1. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. Rose Bengal is a photoreactive compound with its absorbance peaks at 258 nm, 519 nm, and 562 nm Citation 33. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 0 out of 5 $ 9,995 $ 9,495. D. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. 00. SpectraMax ID5 Plate Reader (Molecular Devices) Absorbance. 000 (OD). Read - For the SpectraMax 190 and SpectraMax 340PC384, press to initiate the plate read when you operate the instrument in stand alone mode. SKU: 17247 Category: Laboratory Equip. ®Molecular Devices SpectraMax 190 Microplate Reader. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 0 nm full width half maximum. Plus 384 - SpectraMax Plus 384 Microplate Reader - SpectraMax Plus 384 Absorbance Plate Reader, Molecular Devices - Each . * dsDNA sensitivity specified for the following instruments: SpectraMax® 190 Readers, SpectraMax® Plus384 Readers, SpectraMax® M-Series Readers, SpectraMax® Paradigm® Systems, FlexStation® 3 Systems The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. . Molecular Devices LLC 96 well microplate spectrophotometer spectramax 190 96 Well Microplate Spectrophotometer Spectramax 190, supplied by Molecular Devices LLC, used in various techniques. Absorbance was acquired at 450 nM in the microplate reader SpectraMax 190. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Optical density was measured at 570 nm with an automatic microplate spectrophotometer (SpectraMax 190, Sunnyvale, USA). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Also for: Spectramax plus. Add to Cart Buy. Multi-mode Microplate Readers. SpectraMax 190 Register or Sign In. Monochromator, tunable 1. 00. SpectraMax microplate reader with UV absorbance detection (Molecular Devices) UV–transparent microplates Disposable: Corning cat. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Database contains 2 Molecular Devices SpectraMax 190 Manuals (available for free online viewing or downloading in PDF): Operator's manual, Operation &. TFC values were expressed as rutin equivalents (mg) per gram DW. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 118 Gemini XPS Fluorescence Microplate Reader . SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE READING: FluidX: Perception (Vial. It offers accurate, precise, and flexible assay development tools, such as PathCheck Sensor Interface, PathCheck ABS1 Absorbance Plate, and SpectraTest ABS1 Absorbance Plate. 1step assay with SpectraMax microplate readers. 314 low lamp level warning. DNA standards of known concentrations ranging from 1. Introduction. The plate was mixed on an orbital shaker for 15 s and immediately read using a SpectraMax iD3 (Molecular Devices; San Jose, CA, USA) and an integration time of 400 ms. Italic and boldface type are used for emphasis. Model: SpectraMax 250 Condition: Used.